Role of Nucleotide Excision Repair in Cisplatin Resistance

Nucleotide excision repair gene expression after Cisplatin treatment in melanoma.

Two of the hallmark features of melanoma are its development as a result of chronic UV radiation exposure and the limited efficacy of cisplatin in the disease treatment. Both of these DNA-damaging agents result in large helix-distorting DNA damage that is recognized and repaired by nucleotide excision repair (NER). The aim of this study was to examine the expression of NER gene transcripts, p53...

Nucleotide excision repair pathways involved in Cisplatin resistance in non-small-cell lung cancer.

BACKGROUND In spite of the growing list of genetic abnormalities identified as being involved in DNA repair pathways that alter chemosensitivity in non-small-cell lung cancer (NSCLC) patients, translational assays have not yet been developed for use in individualized chemotherapy. METHODS In metastatic NSCLC, no single cisplatin-based chemotherapy regimen has been shown to be superior to any ...

Novel role of base excision repair in mediating cisplatin cytotoxicity.

Using isogenic mouse embryonic fibroblasts and human cancer cell lines, we show that cells defective in base excision repair (BER) display a cisplatin-specific resistant phenotype. This was accompanied by enhanced repair of cisplatin interstrand cross-links (ICLs) and ICL-induced DNA double strand breaks, but not intrastrand adducts. Cisplatin induces abasic sites with a reduced accumulation in...

Prokaryotic nucleotide excision repair.

Nucleotide excision repair (NER) has allowed bacteria to flourish in many different niches around the globe that inflict harsh environmental damage to their genetic material. NER is remarkable because of its diverse substrate repertoire, which differs greatly in chemical composition and structure. Recent advances in structural biology and single-molecule studies have given great insight into th...

SnapShot: Nucleotide Excision Repair